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PETase ANnotation and Triage System

Nature's solution to a human-made problem, and structural learnings from Nature

PANTS-08c9ad32ce25

Marine plastisphere 245 aa · assembly ERZ21854033_FASTA_predicted_cds · contig ERZ21854033.82661-NODE-82663-length-738-cov-0.912152_1

Retrieval

E-value8.10e-12
Bitscore32.5
Identity to nearest0.305
Nearest characterisedEC:A0A1G8SHF7

Structure

Methodesmfold
Mean pLDDT82.5
RMSD to IsPETase14.57 Å
Aligned fraction0.155

Aligned fraction is a length-normalised gemmi superposition statistic, not a true TM-score.

Active site

TriadS152/D200/H232
Ser–His3.07 Å
His–Asp3.02 Å
Cleft width19.06 Å
Cleft depth3.87 Å

Aromatic clamp: PHE117, PHE159, PHE195, PHE233, PHE64, TRP235, TRP81, TYR113, TYR156, TYR239

Structure, superposed on IsPETase

Catalytic triad drawn as ball-and-stick from the measured residues.

Sequence

IQYQNTTFMKYLLLSFSLFVCLSCPGQENRFDYKKYKNKEGDSLNYRLLTPDANPTRSYPLVIFLHGSGERGGDNEAQLKWGVLQFSGDENMKLYPAFIAAPQCPSDKQRAVYDGRFRAQPTETMALVRAMIDELVTEYPIDRNRIYITGLSMGGYGTFDALTRYPELFAAGVPVCGGVDQSIPDQFNNIPVWIFAGADDDVVPAERSIDMLNALMKEGARPGYTQLPGVGHFSWIAAYSDPVLL