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PETase ANnotation and Triage System

Nature's solution to a human-made problem, and structural learnings from Nature

PANTS-0dc11824c5b8

Human gut 196 aa · assembly ERZ4560756_FASTA_predicted_cds · contig ERZ4560756.5555-Hadza-MoBio-hadza-E-H-G-1-1731--NODE-5555-length-15734-cov-12.908092_15

Retrieval

E-value4.30e-09
Bitscore23.5
Identity to nearest0.279
Nearest characterisedHGMP02

Structure

Methodesmfold
Mean pLDDT85.9
RMSD to IsPETase12.85 Å
Aligned fraction0.189

Aligned fraction is a length-normalised gemmi superposition statistic, not a true TM-score.

Active site

TriadS83/D140/H173
Ser–His3.1 Å
His–Asp2.94 Å
Cleft width19.12 Å
Cleft depth2.73 Å

Aromatic clamp: PHE175, PHE19, PHE21, PHE37, TRP24, TRP36, TYR107, TYR48

Structure, superposed on IsPETase

Catalytic triad drawn as ball-and-stick from the measured residues.

Sequence

ADMTTLKSLAMSGVACYTFDFYGWTDRSTGPQGARWFHDVPRGVDDSYEKKVLQQVEDLNAVIEAAKNWDFVDTNRLYLVGSSMGGATVATAAVTHSDDIRGIVLEYPAINLNPDAMVSGAPLDVNGYTGPVLILQGTKDVIVPMEISQNLTAHYNTLRDDHAELIIYEGQPHVFTGKYKVLAAKEIYRFLEETTS