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PETase ANnotation and Triage System

Nature's solution to a human-made health problem

PANTS-227076ee89fd

Human gut 262 aa · assembly ERZ24772302_FASTA_predicted_cds · contig ERZ24772302.17492-NODE-17492-length-3827-cov-9.151644_2

Retrieval

E-value1.20e-07
Bitscore18.8
Identity to nearest0.272
Nearest characterisedEC:A0ABQ4H167

Structure

Methodesmfold
Mean pLDDT90.5
RMSD to IsPETase13.32 Å
Aligned fraction0.208

Aligned fraction is a length-normalised gemmi superposition statistic, not a true TM-score.

Active site

TriadS160/D209/H241
Ser–His2.96 Å
His–Asp3.1 Å
Cleft width20.47 Å
Cleft depth3.44 Å

Aromatic clamp: PHE123, PHE167, PHE216, PHE248, PHE62, TRP108, TRP244, TYR111, TYR204, TYR236

Structure, superposed on IsPETase

Catalytic triad drawn as ball-and-stick from the measured residues.

Sequence

MKTRTNWLILLIFTFLSTSLYAGDKTSFLKKQFTAQNGYKLNYRILYPLNYSPDKQYPIILFLHGGGEKGSDNESQLLHGGDMFASYENQSNYPAIIIAPQCPEGETWAPYLHPSQNNGKREFPYSAPATKAMKAVKEMLDCYIDKGIVDTKRIYITGLSMGGIGTFDVVCRYPNFFAAASPICGGINLKRLENFKGKTGFSIYHGAADDVVDPQFSRDAYATLQKIHADVRYKEYPGVKHNSWDNAFAEPDYLKWMFQHSL