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PETase ANnotation and Triage System

Nature's solution to a human-made problem, and structural learnings from Nature

PANTS-27b922447349

Landfill 328 aa · assembly ERZ782921_FASTA_CDS_annotated · contig ERZ782921.101794-NODE-101794-length-985-cov-1.170968_1

Retrieval

E-value4.70e-10
Bitscore26.7
Identity to nearest0.364
Nearest characterisedEC:A0ABN3D3P5

Structure

Methodesmfold
Mean pLDDT89.3
RMSD to IsPETase33.04 Å
Aligned fraction0.189

Aligned fraction is a length-normalised gemmi superposition statistic, not a true TM-score.

Active site

TriadS6/D56/H87
Ser–His3.06 Å
His–Asp3.33 Å
Cleft width25.42 Å
Cleft depth4.15 Å

Aromatic clamp: PHE28, PHE51, PHE61, PHE90, PHE95, PHE99, TRP98, TYR52, TYR89, TYR93

Structure, superposed on IsPETase

Catalytic triad drawn as ball-and-stick from the measured residues.

Sequence

FIMGHSMGGGSSHLASSYNVNPRAIISFAAADTDPSAIDAAAEFDGKVVMFYGINDNVTPFDEHQEPIFNNSASTCKTLIGIVGGIHCYFNNYNFNCWFGEVAVGSNAEITREEQQQVVLDFVSLLVQSELHGDSDSHTAYLDSLDNSSRVSVLRECEQSSPEISVVDVNNDVQDITVAFDTPINDAIALLAQQIVVSDTDGGQHNVNLNWTSPTYNQSTAGDYQAIGVFSLPAGVVQSDPPTDLSVNATITVDDPVQTKILSIENIYIYPNPVFDKLSIKSNQIIKTIELYDITGKLVISLYPNLFDMEIDLSVIEDGTYIVKVYTE