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PETase ANnotation and Triage System

Nature's solution to a human-made problem, and structural learnings from Nature

PANTS-289b69ff69ba

Human gut 165 aa · assembly ERZ4560321_FASTA_predicted_cds · contig ERZ4560321.312943-Hadza-MoBio-hadza-E-H-G-21-1740--NODE-312943-length-516-cov-1.895216_1

Retrieval

E-value1.50e-12
Bitscore34.8
Identity to nearest0.328
Nearest characterisedEC:A0A1H5T4B9

Structure

Methodesmfold
Mean pLDDT85.7
RMSD to IsPETase11.50 Å
Aligned fraction0.143

Aligned fraction is a length-normalised gemmi superposition statistic, not a true TM-score.

Active site

TriadS60/D110/H142
Ser–His3.07 Å
His–Asp3.3 Å
Cleft width18.23 Å
Cleft depth4.86 Å

Aromatic clamp: PHE143, TRP29, TRP67, TYR149

Structure, superposed on IsPETase

Catalytic triad drawn as ball-and-stick from the measured residues.

Sequence

SLPRFLQQGQLAPAAYIVMPQLPRDCTSWTDRQTALLALVDHLVSQHQLDNRRVSLTGHSMGGTGTWAVALDHPQRFSAIAPLSGSVRMTREALQSLTSLPVWAVVGAKDTIVPPEASMRFVDALARVNGDCRCTVLPEADHFAVPAVYLDQNTGLLDWLTAQTR