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PETase ANnotation and Triage System

Nature's solution to a human-made problem, and structural learnings from Nature

PANTS-2f33d772d13d

Human gut 287 aa · assembly ERZ24772406_FASTA_predicted_cds · contig ERZ24772406.16025-NODE-16025-length-2301-cov-6.314782_1

Retrieval

E-value7.20e-15
Bitscore42.5
Identity to nearest0.259
Nearest characterisedEC:A0A852TYE9

Structure

Methodesmfold
Mean pLDDT90.8
RMSD to IsPETase10.78 Å
Aligned fraction0.204

Aligned fraction is a length-normalised gemmi superposition statistic, not a true TM-score.

Active site

TriadS127/D223/H255
Ser–His3.16 Å
His–Asp3.17 Å
Cleft width22.57 Å
Cleft depth3.25 Å

Aromatic clamp: PHE171, PHE228, PHE260, PHE261, TRP162, TRP52, TRP58, TYR131, TYR163, TYR173, TYR85, TYR93

Structure, superposed on IsPETase

Catalytic triad drawn as ball-and-stick from the measured residues.

Sequence

MYKRQEKPQYVAITGITYAQVDSWYGHCRRDLKLDLIYPEDFSGKTYPCVVWICGGAWLRMDRSAHFAYLSKLAQSGFVVASVEYRTSNEGEYPMQIQDVKAAIRYLRAHADRYRIDAEYFGVTGESAGGYLTCMAALDEDVSLDVGDYLEQSSRVQAACPWYPPTDTSKFQYQEAEQCAASPESLLLGFNVMKNPEKGYENSPVSKVTKEAPPFLIIHGTNDRTVPFSQSESLYEILEKNGCDVTFLVLEGADHADLQFFQDEVWERMISFFKEKLTRICQEDIKS