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PETase ANnotation and Triage System

Nature's solution to a human-made problem, and structural learnings from Nature

PANTS-34b97219e0df

Human gut 181 aa · assembly ERZ4560700_FASTA_predicted_cds · contig ERZ4560700.180666-Hadza-MoBio-hadza-E-H-E-1-1719--NODE-180666-length-983-cov-1.216336_2

Retrieval

E-value2.40e-12
Bitscore34.2
Identity to nearest0.284
Nearest characterisedEC:A0A243RI45

Structure

Methodesmfold
Mean pLDDT91.2
RMSD to IsPETase10.18 Å
Aligned fraction0.147

Aligned fraction is a length-normalised gemmi superposition statistic, not a true TM-score.

Active site

TriadS81/D129/H160
Ser–His2.96 Å
His–Asp3.15 Å
Cleft width21.89 Å
Cleft depth4.22 Å

Aromatic clamp: PHE166, PHE43, TRP163, TRP48, TYR107, TYR136, TYR167, TYR5

Structure, superposed on IsPETase

Catalytic triad drawn as ball-and-stick from the measured residues.

Sequence

PLVVYLHGSGERGKNNIAQMQRSLLRGIKKHGEPCYIIMPQVFEDKTWTSDGYDAALTRLIEYMLKLYAVDSDRIYITGISMGGAGVLDQLLRHPGKYAAAMPVCGYTDVEDLTAFAGTPMWLAHNSGDNVVSADYSRRIYAAVKDISEAKYTEYDRAGHNAWDRFYSDPEVWNWAFSKTL