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PETase ANnotation and Triage System

Nature's solution to a human-made health problem

PANTS-3a8ed4240aea

Human gut 198 aa · assembly ERZ4561292_FASTA_predicted_cds · contig ERZ4561292.31133-Hadza-MoBio-hadza-A-D-D-16-1621--NODE-31133-length-6995-cov-2.662186_2

Retrieval

E-value1.00e-07
Bitscore19.0
Identity to nearest0.259
Nearest characterisedEC:A0ABT4UC71

Structure

Methodesmfold
Mean pLDDT72.0
RMSD to IsPETase12.37 Å
Aligned fraction0.181

Aligned fraction is a length-normalised gemmi superposition statistic, not a true TM-score.

Active site

TriadS99/D146/H178
Ser–His2.69 Å
His–Asp3.03 Å
Cleft width22.36 Å
Cleft depth4.3 Å

Aromatic clamp: PHE141, PHE18, PHE74, TRP106, TRP181, TRP44, TYR101, TYR103, TYR183, TYR185

Structure, superposed on IsPETase

Catalytic triad drawn as ball-and-stick from the measured residues.

Sequence

MEKERGIIPKTVWLPDVFGNSYGMPQILAKSGIKYFVTHKPCVWNDTNQIPHHTFWWQGPDGLRVFAALSASHFVGTCEPDHIMLNWGRYTDKAKIGESMYCYGTWHTAMARPDLFAAIAPICGGGMAWNAHVLKMPAWVFHGAADETVSVNQSDEMVNAMRANGTDVKYTRMDGVGHGVWDYAYTDEMVKWLLSHQL