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PETase ANnotation and Triage System

Nature's solution to a human-made health problem

PANTS-4427a6e80bbc

Human gut 196 aa · assembly ERZ4560700_FASTA_predicted_cds · contig ERZ4560700.53328-Hadza-MoBio-hadza-E-H-E-1-1719--NODE-53328-length-3013-cov-4.744550_3

Retrieval

E-value3.60e-08
Bitscore20.5
Identity to nearest0.221
Nearest characterisedEC:A0ABW1NJ96

Structure

Methodesmfold
Mean pLDDT92.5
RMSD to IsPETase12.83 Å
Aligned fraction0.166

Aligned fraction is a length-normalised gemmi superposition statistic, not a true TM-score.

Active site

TriadS111/D158/H190
Ser–His3.0 Å
His–Asp2.89 Å
Cleft width25.64 Å
Cleft depth4.12 Å

Aromatic clamp: PHE153, PHE185, PHE35, TRP118, TRP193, TRP80, TYR115, TYR167, TYR50, TYR78

Structure, superposed on IsPETase

Catalytic triad drawn as ball-and-stick from the measured residues.

Sequence

MEEKIYKHPYFGEIYYLLDIPEKYKNTSGAPLLVFLHGAGERGKDHELLYRQGPPRYIREGIKTVDAITLCPQCPTEYIWNDIVIFLKDLIDSIIKEYQIDRTRVSLTGISMGGYGTWEMAMCYPEMFRKIAPVCGGGTPWRAWQIQAKIRAFHGDLDDVVPPESSYQMVDAALNAGKDASLTIFHHVNHGSWDPA