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PETase ANnotation and Triage System

Nature's solution to a human-made problem, and structural learnings from Nature

PANTS-77ec97640668

Human gut 258 aa · assembly ERZ24812776_FASTA_predicted_cds · contig ERZ24812776.8160-NODE-8160-length-2636-cov-7.492832_2

Retrieval

E-value6.30e-12
Bitscore32.8
Identity to nearest0.293
Nearest characterisedEC:A0A8J3U475

Structure

Methodesmfold
Mean pLDDT90.7
RMSD to IsPETase9.79 Å
Aligned fraction0.189

Aligned fraction is a length-normalised gemmi superposition statistic, not a true TM-score.

Active site

TriadS155/D203/H235
Ser–His2.98 Å
His–Asp3.07 Å
Cleft width20.95 Å
Cleft depth3.31 Å

Aromatic clamp: PHE113, PHE198, PHE230, PHE242, PHE58, TRP104, TRP238, TYR103, TYR106, TYR162

Structure, superposed on IsPETase

Catalytic triad drawn as ball-and-stick from the measured residues.

Sequence

MMKQWTTLFVFLFLSLSLSAQQGYGRDIFVSSKGDSLPYRMIHPESVKPGEKYPLVLFLHGAGERGNDNEKQLTHGGQMFLNPVNQEKYPAFVLIPQCPTDGYWAYTERPKSFIPAEMPVGQEISPILQILKQLLDSYLVMPEVDTQRVYIIGLSMGAMGTYDLVIRYPEIFAAAIPICGIVSPSRLSAAKDVKFRIFHGDADDVVPVKGSREAYKALKAAGADVEYIEFPGCNHGSWNPAFNYPGFMDWLFKQKKKR