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PETase ANnotation and Triage System

Nature's solution to a human-made health problem

PANTS-79e49dd125cd

Human gut 213 aa · assembly ERZ4561292_FASTA_predicted_cds · contig ERZ4561292.379-Hadza-MoBio-hadza-A-D-D-16-1621--NODE-379-length-141384-cov-14.638107_33

Retrieval

E-value1.30e-07
Bitscore18.7
Identity to nearest0.288
Nearest characterisedEC:A0A7W3N2I8

Structure

Methodesmfold
Mean pLDDT89.2
RMSD to IsPETase12.85 Å
Aligned fraction0.151

Aligned fraction is a length-normalised gemmi superposition statistic, not a true TM-score.

Active site

TriadS109/D160/H193
Ser–His2.97 Å
His–Asp2.94 Å
Cleft width19.75 Å
Cleft depth4.25 Å

Aromatic clamp: PHE113, PHE194, PHE200, PHE35, PHE77, TRP116, TRP78, TYR155, TYR52

Structure, superposed on IsPETase

Catalytic triad drawn as ball-and-stick from the measured residues.

Sequence

MLKKYYKLNMSDYSNMNYIVYSPETKQNNFPLLLFLHGIGERGKKIEDIEKYALPKYMNRFDIPYIVVAPQCDNNNFWDYHLRDVEKILEKVYKEYKYDKNRVCILGSSMGAFGAWNYIMSRPNLFKGIVSVSGGIMLPIKQTLLPLKEKAILIYHGNIDDVIDVNESIKAYNKLKSIDSKNIELKIIENDNHFLTSHAFKDKYLYEWLERNI