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PETase ANnotation and Triage System

Nature's solution to a human-made problem, and structural learnings from Nature

PANTS-7dd05febb96c

Human gut 155 aa · assembly ERZ4560750_FASTA_predicted_cds · contig ERZ4560750.133188-Hadza-MoBio-hadza-E-H-E-23-1730--NODE-133188-length-1109-cov-1.340116_1

Retrieval

E-value7.80e-12
Bitscore32.5
Identity to nearest0.304
Nearest characterisedEC:A0A8J3U475

Structure

Methodesmfold
Mean pLDDT87.4
RMSD to IsPETase5.65 Å
Aligned fraction0.155

Aligned fraction is a length-normalised gemmi superposition statistic, not a true TM-score.

Active site

TriadS51/D101/H133
Ser–His3.11 Å
His–Asp3.22 Å
Cleft width18.5 Å
Cleft depth5.22 Å

Aromatic clamp: PHE134, PHE96, TRP20, TRP58, TYR141

Structure, superposed on IsPETase

Catalytic triad drawn as ball-and-stick from the measured residues.

Sequence

FGNLRAYVLIPQLPAEQKGWAEVSDQLYELIQNLLKIRGIDRGNISLTGHSMGGTGTWSIGAAYPSLFARIAPLSGSVRSPESTAAKLSDMPVWAFVGSEDTIVPPESSEQTIGWLMELSDQAKITVLEGADHFCVPSLAYLDGERNLVGWLIGG