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PETase ANnotation and Triage System

Nature's solution to a human-made problem, and structural learnings from Nature

PANTS-8031476a0565

Landfill 321 aa · assembly ERZ782921_FASTA_CDS_annotated · contig ERZ782921.78787-NODE-78787-length-1134-cov-3.451344_1

Retrieval

E-value2.40e-08
Bitscore21.1
Identity to nearest0.240
Nearest characterisedEC:D1A646

Structure

Methodesmfold
Mean pLDDT86.5
RMSD to IsPETase15.33 Å
Aligned fraction0.208

Aligned fraction is a length-normalised gemmi superposition statistic, not a true TM-score.

Active site

TriadS190/D267/H296
Ser–His3.05 Å
His–Asp2.89 Å
Cleft width23.53 Å
Cleft depth0.89 Å

Aromatic clamp: PHE119, PHE143, PHE161, PHE209, PHE218, TRP140, TRP189, TRP273, TYR155, TYR158, TYR187, TYR194, TYR36

Structure, superposed on IsPETase

Catalytic triad drawn as ball-and-stick from the measured residues.

Sequence

MKSNYSGKTIIKLNCLLKLKSFIIYSLVLFFGIQSYAIKPNRIYRIYPEKLGLIYRDLNVTTFDGLKIKTWFFPAQPTLSEKEISESWENPIKKPYINIYAENRPTIIIANGDAGNMSFMQLYHAQYFTNKGYNVVTFDWRGFGESSEWEINNDYLVYSEFLIDYDAVIKEVLKQNKEVDTNKIIVYGWSTGAYLSMAAASKYDNIKAFVGIGLITSFEEVMPILKQALKKKEANLIVPDDYPKELQPINIASNFNKSTLLIVGEKDNIAPIWMSEKIFSLLPSKKELWIVEGAEHGGQDGPLKDLDLFHKRVIEFLEKNL