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PETase ANnotation and Triage System

Nature's solution to a human-made problem, and structural learnings from Nature

PANTS-80b626126d56

Human gut 283 aa · assembly ERZ4560750_FASTA_predicted_cds · contig ERZ4560750.1825-Hadza-MoBio-hadza-E-H-E-23-1730--NODE-1825-length-30405-cov-5.934549_9

Retrieval

E-value6.20e-09
Bitscore23.0
Identity to nearest0.284
Nearest characterisedEC:A0A1M6M213

Structure

Methodesmfold
Mean pLDDT85.5
RMSD to IsPETase13.05 Å
Aligned fraction0.215

Aligned fraction is a length-normalised gemmi superposition statistic, not a true TM-score.

Active site

TriadS134/D182/H216
Ser–His3.05 Å
His–Asp2.89 Å
Cleft width24.56 Å
Cleft depth4.35 Å

Aromatic clamp: PHE143, PHE62, TRP105, TRP210, TYR138, TYR79

Structure, superposed on IsPETase

Catalytic triad drawn as ball-and-stick from the measured residues.

Sequence

MKKNTLMILLALACMLIFSTTAKAQYDFLRPVKGEIPGGYDFWVYTPTDYYYSLESTPVVMFLHGASLCSKDMNRSRRYGPLDAIVRGRQIDALVIVPQNPGGSWNPDKLNDILEWAKKNYAMDSTRVYVLGMSLGGYGTMDFAAAYPDKIAAAMALCGGCSAKDKSGLGRLPLWIIHGTGDRAVSVKQSQVVVKELQDTNNDKRLRFDWLRGASHGALARILYMRKTYEWLFSHSTTNPGRPVRPDITIDNSDLTKAYKDMKRGVPSPEIIFEKSIKDDDEY