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PETase ANnotation and Triage System

Nature's solution to a human-made problem, and structural learnings from Nature

PANTS-8731b640e42a

Human gut 262 aa · assembly ERZ24772229_FASTA_predicted_cds · contig ERZ24772229.39354-NODE-39354-length-1221-cov-4.996569_2

Retrieval

E-value2.40e-11
Bitscore30.9
Identity to nearest0.272
Nearest characterisedEC:A0ABV5TJR7

Structure

Methodesmfold
Mean pLDDT89.5
RMSD to IsPETase15.01 Å
Aligned fraction0.264

Aligned fraction is a length-normalised gemmi superposition statistic, not a true TM-score.

Active site

TriadS161/D210/H242
Ser–His3.02 Å
His–Asp3.05 Å
Cleft width20.3 Å
Cleft depth3.6 Å

Aromatic clamp: PHE168, PHE217, PHE237, PHE249, PHE62, TRP108, TRP245, TYR111, TYR124, TYR205

Structure, superposed on IsPETase

Catalytic triad drawn as ball-and-stick from the measured residues.

Sequence

MKTLRSIFFLSLCLLLSVAGYAGDKASFLKKQFTARDGYQLNYRVLYPRDYNPAQKYPVILFLHGAGERGSDNEAQLIHGGDMFASFENQTKYPAIIIAPQCPAEKTWSEYKGLNAGKEGKRFYPLNAPATQSMAAVKELLDSYIAEGKVDTKRIYVTGLSMGGMGTFDIVMRYPNLFAAATPICGGANLQRLANFKGKTAFSIYHGGSDSVVDVQFSRDANEALKKAGADVRYKEFPGVDHNSWDNAFAEPDYLAWMFQHS