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PETase ANnotation and Triage System

Nature's solution to a human-made problem, and structural learnings from Nature

PANTS-a306063fcbaf

Human gut 255 aa · assembly ERZ24772302_FASTA_predicted_cds · contig ERZ24772302.672-NODE-672-length-42953-cov-13.365681_21

Retrieval

E-value7.80e-12
Bitscore32.5
Identity to nearest0.333
Nearest characterisedEC:A0A5N6AZJ9

Structure

Methodesmfold
Mean pLDDT92.9
RMSD to IsPETase11.04 Å
Aligned fraction0.174

Aligned fraction is a length-normalised gemmi superposition statistic, not a true TM-score.

Active site

TriadS154/D202/H234
Ser–His2.93 Å
His–Asp3.14 Å
Cleft width20.7 Å
Cleft depth3.53 Å

Aromatic clamp: PHE112, PHE161, PHE197, PHE229, PHE241, PHE57, TRP103, TRP237, TYR102, TYR105

Structure, superposed on IsPETase

Catalytic triad drawn as ball-and-stick from the measured residues.

Sequence

MKRLISAICVLFFLLPLSAQETYQKETFISSRGDTLQYRLLQPENMKKSEKYPLVLFLHGAGERGNDNERQLTHGGQMFLNPVNREKYPAFVLAPQCPETGYWAYSARPESFLPNEMPLNEPITPIFQTVKDLLDTYLAMPQVDKSRIYIIGLSMGGMGTFDMAVRFPEIFAAAVPICGTVNVARLKAVKSVKFRIFHGDADNVVTPEGSRAAYRTLKKEGADVEYIEFPGCNHGSWNPAFNYPDFMSWLFSQKK