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PETase ANnotation and Triage System

Nature's solution to a human-made problem, and structural learnings from Nature

PANTS-ad3bc58e12cd

Human gut 244 aa · assembly ERZ4560750_FASTA_predicted_cds · contig ERZ4560750.119900-Hadza-MoBio-hadza-E-H-E-23-1730--NODE-119900-length-1222-cov-11.883843_1

Retrieval

E-value2.30e-10
Bitscore27.7
Identity to nearest0.289
Nearest characterisedEC:A0A5S4FJI0

Structure

Methodesmfold
Mean pLDDT90.7
RMSD to IsPETase19.41 Å
Aligned fraction0.196

Aligned fraction is a length-normalised gemmi superposition statistic, not a true TM-score.

Active site

TriadS140/D190/H222
Ser–His2.88 Å
His–Asp3.22 Å
Cleft width17.44 Å
Cleft depth4.46 Å

Aromatic clamp: PHE223, TRP111, TRP147, TYR229, TYR64

Structure, superposed on IsPETase

Catalytic triad drawn as ball-and-stick from the measured residues.

Sequence

MELTRRGFLSLTAASAFCLLCAGCGDAAAVSPAGTLTAHVGDAGLSYWLYTPQSTGTPKTLLLYLHGGSGKGNDLNAVLEAESLPRFLQERQLAPDAYVVMPQLPASCKGWSDRTAQLLQLLATIKAQYPIARTALTGHSMGGTGCWDLAVAHPEAFCAAAPLSGSVQLTEQNLTALQSLPVWAVVGSADTIVKPDASEQFIAALKEKNPNARLTVREGATHFDVPQAYLDDALGLLDWLTAAR