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PETase ANnotation and Triage System

Nature's solution to a human-made problem, and structural learnings from Nature

PANTS-adc06bbd1acb

Human gut 281 aa · assembly ERZ4226326_FASTA_predicted_cds · contig ERZ4226326.24398-NODE-24398-length-851-cov-2.042714_1

Retrieval

E-value6.00e-11
Bitscore29.6
Identity to nearest0.314
Nearest characterisedPLC:A0A163MA58

Structure

Methodesmfold
Mean pLDDT90.7
RMSD to IsPETase8.46 Å
Aligned fraction0.196

Aligned fraction is a length-normalised gemmi superposition statistic, not a true TM-score.

Active site

TriadS128/D189/H221
Ser–His3.13 Å
His–Asp2.83 Å
Cleft width14.51 Å
Cleft depth3.61 Å

Aromatic clamp: TRP168, TYR127, TYR135, TYR170

Structure, superposed on IsPETase

Catalytic triad drawn as ball-and-stick from the measured residues.

Sequence

YNDPGKIEQRYRWHGPYEVKSVVLSDGEAKYGNYKYKVWYPVQQAGRERPRPLVITLNGTGGSCDKDEPIFRHLASWGFVVAGNTDAQTAPGFSAEWTLDQALAANQDSKSPLYHQIDEKEIGIVGYSQGSAGAYNTLEGKDGDKFKTMVTVSGVTESIGEKLHLPVWIYDPSKVTISVFMAAGTGILDRKLITPLDEMKENYSKIQSKTAAMGRRKDADHLDIQTAADAYITAWLRWQLDDDIYAKRVFTGKDPEILRNPAWDNAELRNPETTQPKQTEK