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PETase ANnotation and Triage System

Nature's solution to a human-made problem, and structural learnings from Nature

PANTS-b976dd3ca628

Human gut 291 aa · assembly ERZ4560928_FASTA_predicted_cds · contig ERZ4560928.5669-Hadza-MoBio-C-REDO-E-3-1554--NODE-5669-length-7376-cov-2.880258_8

Retrieval

E-value3.50e-15
Bitscore43.5
Identity to nearest0.314
Nearest characterisedEC:A0ABW2Y2H3

Structure

Methodesmfold
Mean pLDDT91.1
RMSD to IsPETase11.95 Å
Aligned fraction0.189

Aligned fraction is a length-normalised gemmi superposition statistic, not a true TM-score.

Active site

TriadS131/D227/H259
Ser–His3.16 Å
His–Asp3.26 Å
Cleft width22.63 Å
Cleft depth3.18 Å

Aromatic clamp: PHE175, PHE232, PHE264, PHE265, TRP166, TRP56, TRP62, TYR135, TYR167, TYR177, TYR89, TYR97

Structure, superposed on IsPETase

Catalytic triad drawn as ball-and-stick from the measured residues.

Sequence

MKEVLKINVEKPQYVTITGITYAQVDSWYGHCRRDLKLDLIYPEDFSGKTYPCVVWICGGAWLRMDRSAHLAYLSKLAQSGFVVASVEYRTSNEGEYPMQIQDVKAAIRYLRAHADRYRIDAEYIGVTGESAGGYLTCMAALDEAVSLDVGDYLEQSSKVQAACPWYPPTDTSKFPYQNAEQCAASPESLLLGFNVMRNPKKGYENSPVSKVTKDAPPFLIIHGMNDHTVPFSQSESLYETLEANGCDVTLLALEGADHADLQFFQDKIWERMISFFKEKLTRICQENIKS