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PETase ANnotation and Triage System

Nature's solution to a human-made problem, and structural learnings from Nature

PANTS-bca3c3c4cafb

Human gut 281 aa · assembly ERZ4560321_FASTA_predicted_cds · contig ERZ4560321.17148-Hadza-MoBio-hadza-E-H-G-21-1740--NODE-17148-length-7009-cov-59.195326_2

Retrieval

E-value2.10e-09
Bitscore24.6
Identity to nearest0.224
Nearest characterisedHGMP04

Structure

Methodesmfold
Mean pLDDT85.7
RMSD to IsPETase15.81 Å
Aligned fraction0.211

Aligned fraction is a length-normalised gemmi superposition statistic, not a true TM-score.

Active site

TriadS168/D225/H258
Ser–His3.05 Å
His–Asp3.01 Å
Cleft width20.18 Å
Cleft depth2.72 Å

Aromatic clamp: PHE104, PHE106, PHE122, PHE260, TRP109, TRP121, TYR133, TYR192, TYR76, TYR84

Structure, superposed on IsPETase

Catalytic triad drawn as ball-and-stick from the measured residues.

Sequence

MKKWIKAVLVGAGAFAVVAGIAAVIGIGHYVGFPKPAGEEKTGYRTEKLCVEYDGISLYGKALIPDGDGPFSTVLYAHGAESDYKADMTTLKSLAMSGVACYTFDFYGWTDRSTGPQGVHWFHDVPRGVDDSYEKKVLQQVEDLNAVIEAAKGWDFVDTSRLYLVGSSMGGATVATAAVTHSDDIRGIVLQYPAINLNPDAMVSGAPLDVNGYTGPVLILQGTKDVIVPMEMSQDLTAHYNTLRDDHAELILYEGQPHVFMGKYKVLAAKEIYRFLKEVAS