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PETase ANnotation and Triage System

Nature's solution to a human-made problem, and structural learnings from Nature

PANTS-bd638266b6e3

Human gut 137 aa · assembly ERZ4560321_FASTA_predicted_cds · contig ERZ4560321.143624-Hadza-MoBio-hadza-E-H-G-21-1740--NODE-143624-length-1029-cov-1.473739_1

Retrieval

E-value1.60e-12
Bitscore34.8
Identity to nearest0.365
Nearest characterisedEC:A0A8J3U475

Structure

Methodesmfold
Mean pLDDT90.4
RMSD to IsPETase11.06 Å
Aligned fraction0.151

Aligned fraction is a length-normalised gemmi superposition statistic, not a true TM-score.

Active site

TriadS34/D84/H116
Ser–His3.1 Å
His–Asp3.22 Å
Cleft width18.94 Å
Cleft depth6.52 Å

Aromatic clamp: PHE117, PHE79, TRP3, TRP41

Structure, superposed on IsPETase

Catalytic triad drawn as ball-and-stick from the measured residues.

Sequence

RGWSDMDWVLMQLISAAVREYDLDSANVSLTGHSMGGTGVWAIAAAHPGTFARIAPLSGSIRKTDENLEALKWVPVYAFVGDADTIVPPESSEEFISALQDQGSNARIEILEGADHFAVPGLAYLGGYGLVDWLIGN