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PETase ANnotation and Triage System

Nature's solution to a human-made problem, and structural learnings from Nature

PANTS-c0f45e49472f

Human gut 260 aa · assembly ERZ4560756_FASTA_predicted_cds · contig ERZ4560756.147057-Hadza-MoBio-hadza-E-H-G-1-1731--NODE-147057-length-923-cov-1.536643_2

Retrieval

E-value2.90e-11
Bitscore30.7
Identity to nearest0.287
Nearest characterisedEC:A0ABR9P363

Structure

Methodesmfold
Mean pLDDT91.5
RMSD to IsPETase7.91 Å
Aligned fraction0.208

Aligned fraction is a length-normalised gemmi superposition statistic, not a true TM-score.

Active site

TriadS119/D201/H233
Ser–His2.85 Å
His–Asp3.15 Å
Cleft width9.52 Å
Cleft depth3.54 Å

Aromatic clamp: PHE123, PHE151, TYR120, TYR167

Structure, superposed on IsPETase

Catalytic triad drawn as ball-and-stick from the measured residues.

Sequence

LVDAELCHYTSFDGLTVPYWYYKAPGKQEGPRPVLIDIHGGPEGQERPMFAALTEYLVSQGMNIIAPNVRGSVGYGKTYTHLDDVEKRLDSVHDIDALVKHLIETGIADKDRIAVMGGSYGGFMTLSCVARYPDLWAAGVDVVGMTNLVTFLENTSEYRRAHRESEYGTLAHDRETLYNVSPIAKVGDIKAPLMVIHGANDPRVPVTEADMIVENVKSRGIEVSYLRYEDEGHGLAKRKNQLDCYPQVVAFLKKHLRMED