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PETase ANnotation and Triage System

Nature's solution to a human-made problem, and structural learnings from Nature

PANTS-d5ffa53ac4f9

Human gut 184 aa · assembly ERZ4560895_FASTA_predicted_cds · contig ERZ4560895.91465-Hadza-MoBio-hadza-A-D-J-9-1548--NODE-91465-length-552-cov-1.665263_1

Retrieval

E-value7.90e-12
Bitscore32.5
Identity to nearest0.327
Nearest characterisedEC:A0A8J3U475

Structure

Methodesmfold
Mean pLDDT93.9
RMSD to IsPETase10.61 Å
Aligned fraction0.155

Aligned fraction is a length-normalised gemmi superposition statistic, not a true TM-score.

Active site

TriadS95/D145/H177
Ser–His2.95 Å
His–Asp3.15 Å
Cleft width17.7 Å
Cleft depth4.41 Å

Aromatic clamp: PHE140, PHE178, PHE36, TRP102, TRP64, TYR16

Structure, superposed on IsPETase

Catalytic triad drawn as ball-and-stick from the measured residues.

Sequence

LYTPTDPQQNMPLIVYLHGGSGKGDDLNLITAVDGFPSYLQSGSFGNLRAYVLIPQLPAEQKGWAEVSDQLYELIQNLLETRGIDRGNISLTGHSMGGTGTWSMGAAYPSLFARIAPLSGSVRSPESTAAKLSDTPVWAFVGSEDTIVPPESSEQTIGWLMELSDQAKITVLEGADHFSVPSLA