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PETase ANnotation and Triage System

Nature's solution to a human-made problem, and structural learnings from Nature

PANTS-d9f57891fa57

Human gut 273 aa · assembly ERZ4560321_FASTA_predicted_cds · contig ERZ4560321.39132-Hadza-MoBio-hadza-E-H-G-21-1740--NODE-39132-length-3459-cov-3.026907_3

Retrieval

E-value4.20e-11
Bitscore30.1
Identity to nearest0.333
Nearest characterisedPLC:A0A147KJY8

Structure

Methodesmfold
Mean pLDDT89.5
RMSD to IsPETase15.06 Å
Aligned fraction0.185

Aligned fraction is a length-normalised gemmi superposition statistic, not a true TM-score.

Active site

TriadS169/D219/H251
Ser–His2.9 Å
His–Asp3.11 Å
Cleft width17.77 Å
Cleft depth4.38 Å

Aromatic clamp: PHE110, PHE214, PHE252, TRP138, TRP176, TYR90

Structure, superposed on IsPETase

Catalytic triad drawn as ball-and-stick from the measured residues.

Sequence

MKQKIHLMITMLMVSTVLLCGCHNKTENYFDETKSNVTDDFATEKEKTAPSEHTSASFTSCSINTSQLGTFRYWLYTPSNPTKNMPLIVYLHGGSEKGDDLNKITSVDGFPKYLQSGQLKNVRAYVIIPQLPSTQKSWEGASEAIYDLIDQTISTYKINKNNISLTGHSMGGTGTWDIACTYPELFARIAPLSGSIHNTPYVINKLKNISVRAFVGSADTIVSPDSSVEVVEALKAVGADAEITEFDGADHFSVPSLTFLDTNIDLVDWLICG