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PETase ANnotation and Triage System

Nature's solution to a human-made problem, and structural learnings from Nature

PANTS-e03b1a21a631

Human gut 286 aa · assembly ERZ4560756_FASTA_predicted_cds · contig ERZ4560756.21312-Hadza-MoBio-hadza-E-H-G-1-1731--NODE-21312-length-5300-cov-52.319740_4

Retrieval

E-value1.70e-10
Bitscore28.2
Identity to nearest0.303
Nearest characterisedEC:A0ABW1CGM7

Structure

Methodesmfold
Mean pLDDT83.3
RMSD to IsPETase15.49 Å
Aligned fraction0.185

Aligned fraction is a length-normalised gemmi superposition statistic, not a true TM-score.

Active site

TriadS170/D220/H252
Ser–His2.96 Å
His–Asp3.13 Å
Cleft width19.23 Å
Cleft depth4.42 Å

Aromatic clamp: PHE111, PHE215, PHE253, TRP139, TRP177, TYR227, TYR91

Structure, superposed on IsPETase

Catalytic triad drawn as ball-and-stick from the measured residues.

Sequence

MKSKWIRAGATVLLMAGLLGTGYIESHKERALNTIDASAIATTLSAELGSENEEVPAQLLMCSTSTSQIETFRYWLYTPKNPVENMPLIVYLHGSSGKGDDLNILMNEDGFPQYLKNGQLGDIPAYILIPQLPAEKRGWASVSQSVYQLISETVSTCQIDSDNISLTGHSMGGTGTWNLAAQYPQMFARIAPLSGSARGQAAKLEALKEIPVRAFVGSDDTVILPEYSESAVSELNALGGNAQITIFEDADHFAVPSQTYLDPSISLVNWLIGNDAEKENDNETVL