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PETase ANnotation and Triage System

Nature's solution to a human-made problem, and structural learnings from Nature

PANTS-e16be9b9b0d7

Human gut 244 aa · assembly ERZ4560750_FASTA_predicted_cds · contig ERZ4560750.100694-Hadza-MoBio-hadza-E-H-E-23-1730--NODE-100694-length-1438-cov-42.096988_2

Retrieval

E-value4.20e-10
Bitscore26.8
Identity to nearest0.291
Nearest characterisedEC:A0A1I0JU31

Structure

Methodesmfold
Mean pLDDT90.6
RMSD to IsPETase17.59 Å
Aligned fraction0.192

Aligned fraction is a length-normalised gemmi superposition statistic, not a true TM-score.

Active site

TriadS140/D190/H222
Ser–His2.86 Å
His–Asp3.17 Å
Cleft width17.35 Å
Cleft depth4.58 Å

Aromatic clamp: PHE223, TRP111, TRP147, TYR229, TYR64

Structure, superposed on IsPETase

Catalytic triad drawn as ball-and-stick from the measured residues.

Sequence

MELTRRDFLSLTAASAFCLLCVGCGDASAVSPAGALTAHTGDAGLSYWLYTPQSTGTPKTLVLYLHGGSGKGSNLNAVLEAESLPRFLQEGQLAPDAYVVMPQLPANCKGWSDCTAQLMQLLAVIKAQYPIARTALTGHSMGGTGCWDLAVAYPETFCAAAPLSGSVRLTEQNLTALQSLPVWAVVGSADTIVKPDDSEQFITALQEKNPNAQLTVREGATHFDVPQAYLDSTLGLLDWLTAAR