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PETase ANnotation and Triage System

Nature's solution to a human-made health problem

PANTS-e9f9026fb0e6

Human gut 250 aa · assembly ERZ4560700_FASTA_predicted_cds · contig ERZ4560700.5630-Hadza-MoBio-hadza-E-H-E-1-1719--NODE-5630-length-18461-cov-3.351773_14

Retrieval

E-value5.20e-07
Bitscore16.7
Identity to nearest0.247
Nearest characterisedEC:A0A8J3U475

Structure

Methodesmfold
Mean pLDDT89.3
RMSD to IsPETase13.65 Å
Aligned fraction0.181

Aligned fraction is a length-normalised gemmi superposition statistic, not a true TM-score.

Active site

TriadS147/D195/H227
Ser–His3.02 Å
His–Asp2.94 Å
Cleft width19.2 Å
Cleft depth3.74 Å

Aromatic clamp: PHE151, PHE190, PHE234, PHE53, TRP104, TRP154, TRP230, TRP99, TYR109

Structure, superposed on IsPETase

Catalytic triad drawn as ball-and-stick from the measured residues.

Sequence

MRECNNTAGFDVSRAFGFFTYRMGDLEIPYRFYVPAAVNKSESANAPRPLVIFLHGSGSRGNDNTAPLENVLAKAFLDPENPIFASYVLAPQCGSGRQWVNTPWAKGNYSVDATPESPELAAVIELTKKLCRENNIDRGRIYLTGVSMGGFGVWDMLARHGELFAYAMPVCGGGDPSYARKMKDIPICTFHGSADESVPVSGTREMVEAVKAAGGKITYTELKDAPHAIWDGVFSDTSNFIRMFAQTKNP