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PETase ANnotation and Triage System

Nature's solution to a human-made health problem

PANTS-ec1db9554425

Human gut 188 aa · assembly ERZ4239636_FASTA_predicted_cds · contig ERZ4239636.122-NODE-122-length-32966-cov-14.885601_18

Retrieval

E-value1.00e-05
Bitscore12.5
Identity to nearest0.251
Nearest characterisedPLC:W7JED3

Structure

Methodesmfold
Mean pLDDT89.7
RMSD to IsPETase14.01 Å
Aligned fraction0.143

Aligned fraction is a length-normalised gemmi superposition statistic, not a true TM-score.

Active site

TriadS94/D141/H172
Ser–His3.24 Å
His–Asp3.4 Å
Cleft width23.38 Å
Cleft depth2.83 Å

Aromatic clamp: PHE175, PHE35, PHE39, PHE48, PHE51, PHE6, TRP135, TRP50, TYR121, TYR134, TYR143, TYR152, TYR17, TYR67

Structure, superposed on IsPETase

Catalytic triad drawn as ball-and-stick from the measured residues.

Sequence

MTTLVFLHGSGADKNAYFETMQNIASKFNAQLITFNAPFPHPNKKGKFVWFNKKEINGRRDAIEEEYFHSLQYVKELLTKLKNDDDDIILIGHSQGGGLAVHLGLEMELKIVVSICGDLPYNISYANNSTTPIYWCEGGKDTYIDNNRKASYLFLKEIRANYHYQILPNSTHNEFTADLLNLIESIKV