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PETase ANnotation and Triage System

Nature's solution to a human-made problem, and structural learnings from Nature

PANTS-eed0edd3ca26

Human gut 268 aa · assembly ERZ4560321_FASTA_predicted_cds · contig ERZ4560321.1707-Hadza-MoBio-hadza-E-H-G-21-1740--NODE-1707-length-39562-cov-19.346309_7

Retrieval

E-value6.60e-09
Bitscore22.9
Identity to nearest0.329
Nearest characterisedEC:A0A239AX86

Structure

Methodesmfold
Mean pLDDT88.8
RMSD to IsPETase11.95 Å
Aligned fraction0.200

Aligned fraction is a length-normalised gemmi superposition statistic, not a true TM-score.

Active site

TriadS124/D172/H206
Ser–His3.03 Å
His–Asp2.89 Å
Cleft width20.37 Å
Cleft depth4.34 Å

Aromatic clamp: PHE133, PHE212, PHE213, PHE254, PHE52, TRP95, TYR128, TYR69

Structure, superposed on IsPETase

Catalytic triad drawn as ball-and-stick from the measured residues.

Sequence

MLTLILSASLQTKAQYDFIRPVKDAIPGGYNFWVYTPTEYYYTLNETPVIIFLHGQSLCGRDLNRVRRYGPLDAIVKGRQVDALVVVPQNPGGAWNPKKINDVLEWTKQNYACDSTRVYVIGMSLGGYGTLDFACTYPDKVAAALAMCGGCSKKDRTPLGKLPLWIIHGTADRAVSISQSKSVVNDLEKSGNDSRLRYTWLQGANHGAPARFFYMRKTYNWLFSHSLLDPDRPVNKEITLDMSDLPKAYRDMDFGKADPETVYETTIK