########     ###    ##    ## ########  ######  
##     ##   ## ##   ###   ##    ##    ##    ## 
##     ##  ##   ##  ####  ##    ##    ##       
########  ##     ## ## ## ##    ##     ######  
##        ######### ##  ####    ##          ## 
##        ##     ## ##   ###    ##    ##    ## 
##        ##     ## ##    ##    ##     ######  

PETase ANnotation and Triage System

Nature's solution to a human-made problem, and structural learnings from Nature

PANTS-f1bc07683fad

Marine plastisphere 339 aa · assembly ERZ21854028_FASTA_predicted_cds · contig ERZ21854028.52759-NODE-52766-length-1028-cov-1.970195_1

Retrieval

E-value1.10e-21
Bitscore64.9
Identity to nearest0.258
Nearest characterisedEC:A0A8H9GVK2

Structure

Methodesmfold
Mean pLDDT84.7
RMSD to IsPETase10.30 Å
Aligned fraction0.219

Aligned fraction is a length-normalised gemmi superposition statistic, not a true TM-score.

Active site

TriadS93/D185/H216
Ser–His3.04 Å
His–Asp3.12 Å
Cleft width21.7 Å
Cleft depth1.83 Å

Aromatic clamp: PHE14, PHE165, PHE217, PHE230, PHE236, PHE58, PHE94, TYR9, TYR97

Structure, superposed on IsPETase

Catalytic triad drawn as ball-and-stick from the measured residues.

Sequence

VVLSHGSGYDRTVFRYLAERLASHGFVALAVEHPDSSAEQINALLTGSSAMVVPDDEFLERPRKITALLDYLEAQPVWQQQIDFDRIGVLGQSFGGYTGLALAGAQLNFEQLAAECPPGTLSLNISLLLQCQALELADPGATTLALGDARVKAAIAINPITSVLFGESSLSQIEAPTLILAGGADTVAPALSEQIRPYTWLTMPERYLMVMPLGTHFSVSAASETGSEAFVLPPQFLGQRPDIDQAYVAATSLAFFETYLEVGTQFRPLLSSAGTIALSDQLSQRTIPLWLLQNFTADTLRASLTQPNIAVEAGIAPNALLGSDSASAATIEAAPFAAP