HGMP03
Bacteroidia: Porphyromonadaceae · 323 aa · HGMP-measured
Structure, superposed on IsPETase
Aligned onto IsPETase 6EQE when it was written, so anything added below overlays directly and the browser does no alignment. The catalytic triad is drawn from the residues the geometry stage measured, not from positions inferred by an alignment.
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Sequence 323 aa
catalytic triad · the same colours as the viewer above. Triad positions are labelled; hover any residue for its number. Click one to select it in both.
Activity
| Optimum temperature | not recorded |
| Optimum pH | not recorded |
| Family | petase_like |
Structure
| Source | ESMFold prediction |
| Mean pLDDT | 79.7 |
| Residues | 323 |
| Cα RMSD to IsPETase | 9.50 Å |
Active site
| Catalytic triad | Ser152 · His250 · Asp220 |
| Ser OG → His NE2 | 2.89 Å |
| His ND1 → Asp OD | 2.99 Å |
| Oxyanion donor 1 | 153 (2.89 Å) |
| Oxyanion donor 2 | 80 (4.51 Å) |
| Cleft width | 23.31 Å |
| Cleft depth | 3.61 Å |
| Cleft residues | 81 |
Aromatic clamp: PHE113 · PHE160 · PHE189 · PHE231 · TRP115 · TRP253 · TRP80 · TYR104 · TYR183 · TYR229 · TYR83
Measured activity
| Parameter | Value | Substrate | Evidence | Source |
|---|---|---|---|---|
| ordinal activity | — | PET nanoparticle | measured | PMID 39551294 · 10.1016/j.ijbiomac.2024.137732 |
| below HGMP01; not ranked against the other three. Assay: 0.5 mg/mL PET nanoparticles in 200 uL, 50 mM sodium phosphate (pH 6-8) or glycine-NaOH (pH 9-11), 25-45 degC, 24 h. | ||||
Related in PANTS
Lineage
No recorded parent or derived variants.
Nearest metagenomic candidates
No candidate in the catalogue names this enzyme as its nearest match.
Identifiers and cross-references
Activity
Human gut PET hydrolase, measured. Deposit id GUT_GENOME137663_00143 (the paper's HGMP03 is HGMP06 in the supplementary). 96 homologues in UHGP-100. DLH domain: no. PMID 39551294.