MHETase
Piscinibacter sakaiensis · 603 aa · UniProt
Structure, superposed on IsPETase
Aligned onto IsPETase 6EQE when it was written, so anything added below overlays directly and the browser does no alignment. The catalytic triad is drawn from the residues the geometry stage measured, not from positions inferred by an alignment.
Overlay another
Sequence 603 aa
catalytic triad · the same colours as the viewer above. Triad positions are labelled; hover any residue for its number. Click one to select it in both.
Activity
| Optimum temperature | not recorded |
| Optimum pH | not recorded |
| Family | mhetase_like |
Structure
| Source | Experimental, PDB 6QGA |
| Resolution | 2.1 Å |
| Residues | 558 |
| Cα RMSD to IsPETase | 21.39 Å |
Active site
| Catalytic triad | Ser225 · His528 · Asp492 |
| Ser OG → His NE2 | 2.77 Å |
| His ND1 → Asp OD | 3.06 Å |
| Oxyanion donor 1 | 226 (3.11 Å) |
| Oxyanion donor 2 | 132 (5.74 Å) |
| Cleft width | 24.28 Å |
| Cleft depth | 1.94 Å |
| Cleft residues | 102 |
Aromatic clamp: PHE221 · PHE415 · PHE424 · PHE495 · TRP376 · TRP394 · TRP397 · TRP398 · TRP420 · TYR194 · TYR252 · TYR373 · TYR487
Measured activity
No measurement rows. The enzyme is in the reference set on the strength of its curation source rather than a value extracted into this database.
Related in PANTS
Lineage
No recorded parent or derived variants.
Nearest metagenomic candidates
No candidate in the catalogue names this enzyme as its nearest match.
Identifiers and cross-references
Sequence
Ideonella/Piscinibacter sakaiensis. Tannase/feruloyl esterase group with a lid domain, NOT the PETase family: a PETase-seeded profile search never reaches it (spec section 2, point 5). 603 aa.