Micpa-PETase
Micromonospora pattaloongensis · 294 aa · PAZy-measured
Structure, superposed on IsPETase
Aligned onto IsPETase 6EQE when it was written, so anything added below overlays directly and the browser does no alignment. The catalytic triad is drawn from the residues the geometry stage measured, not from positions inferred by an alignment.
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Sequence 294 aa
catalytic triad · the same colours as the viewer above. Triad positions are labelled; hover any residue for its number. Click one to select it in both.
Activity
| Optimum temperature | not recorded |
| Optimum pH | not recorded |
| Family | petase_like |
Structure
| Source | Experimental, PDB 8YTU |
| Resolution | 1.34 Å |
| Residues | 250 |
| Cα RMSD to IsPETase | 1.12 Å |
Active site
| Catalytic triad | Ser168 · His245 · Asp214 |
| Ser OG → His NE2 | 2.88 Å |
| His ND1 → Asp OD | 3.16 Å |
| Oxyanion donor 1 | 169 (3.09 Å) |
| Oxyanion donor 2 | 98 (5.21 Å) |
| Cleft width | 25.43 Å |
| Cleft depth | 4.33 Å |
| Cleft residues | 82 |
Aromatic clamp: PHE105 · PHE128 · PHE247 · TRP102 · TRP193 · TYR227 · TYR98
Measured activity
| Parameter | Value | Substrate | Evidence | Source |
|---|---|---|---|---|
| product release | 1506.159546083895 uM | PET film | measured | 10.1126/science.adp5637 |
| product release 1506 +/- 339 uM (5 replicates) | ||||
| tm | 68.44 degC | — | measured | 10.1126/science.adp5637 |
| melting temperature 68.44 degC | ||||
Related in PANTS
Lineage
No recorded parent or derived variants.
Nearest metagenomic candidates
No candidate in the catalogue names this enzyme as its nearest match.
Identifiers and cross-references
Structure
Sequence
Library entry 1428 (SDZ16714.1) in the Science 2025 PET depolymerase landscape. Product release 1.51e+03 +/- 339 uM, classed ACTIVE against its own replicate noise (active above 2 SD, inactive at or below 1 SD). Source: Landscape profiling of PET depolymerases using a natural sequence cluster framework, Science 2025 (doi:10.1126/science.adp5637), Data S3.