BhrPETase
293 aa · UniProt
Structure, superposed on IsPETase
Aligned onto IsPETase 6EQE when it was written, so anything added below overlays directly and the browser does no alignment. The catalytic triad is drawn from the residues the geometry stage measured, not from positions inferred by an alignment.
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Sequence 293 aa
catalytic triad · the same colours as the viewer above. Triad positions are labelled; hover any residue for its number. Click one to select it in both.
Activity
| Optimum temperature | not recorded |
| Optimum pH | not recorded |
| Family | petase_like |
Structure
| Source | AlphaFold model, A0A2H5Z9R5 |
| Mean pLDDT | 92.0 |
| Residues | 293 |
| Cα RMSD to IsPETase | 1.51 Å |
Active site
| Catalytic triad | Ser165 · His242 · Asp210 |
| Ser OG → His NE2 | 2.88 Å |
| His ND1 → Asp OD | 3.13 Å |
| Oxyanion donor 1 | 166 (2.95 Å) |
| Oxyanion donor 2 | 95 (4.52 Å) |
| Cleft width | 17.03 Å |
| Cleft depth | 4.44 Å |
| Cleft residues | 83 |
Aromatic clamp: PHE127 · PHE222 · PHE243 · TRP190 · TYR95
Measured activity
No measurement rows. The enzyme is in the reference set on the strength of its curation source rather than a value extracted into this database.
Related in PANTS
Lineage
Variants built on this enzyme:
TurboPETase
Nearest metagenomic candidates
No candidate in the catalogue names this enzyme as its nearest match.
Identifiers and cross-references
Structure
Sequence
Bacterium HR29 PET hydrolase, 293 aa. Added as a wild type because it is TurboPETase's parent, and a variant can only be derived from a parent that lives in WILD_TYPES. It is also a PAZy-measured positive in its own right (PAZy:17), so it duplicates that row: the same already holds for IsPETase, LCC and Cut190, and is harmless because evaluation splits by 30% cluster, which keeps identical sequences on the same side. NO LIVE UNIPROTKB ENTRY: A0A2H5Z9R5, the accession PAZy still records, is inactive (DEMERGED to A0ACD6B9U1), and A0ACD6B9U1 is itself DELETED as 'not part of a reference proteome'. The sequence therefore comes from UniParc, which never deletes, and is confirmed three ways: it is byte-identical to PAZy:17, it carries an active EMBL WGS cross-reference (GBD22443, 'Poly(Ethylene terephthalate) hydrolase'), and all eight TurboPETase mutations match it at offset 0.