HotPETase
272 aa · engineered from IsPETase · PDB-construct
21 mutations; melting temperature 82.5 °C
Structure, superposed on IsPETase
Aligned onto IsPETase 6EQE when it was written, so anything added below overlays directly and the browser does no alignment. The catalytic triad is drawn from the residues the geometry stage measured, not from positions inferred by an alignment.
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Sequence 272 aa
catalytic triad · the same colours as the viewer above. Triad positions are labelled; hover any residue for its number. Click one to select it in both.
Activity
| Optimum temperature | 62.5 °C |
| As published | Published as 60 to 65 °C. Midpoint stored in the numeric column; the published interval is this text. |
| Optimum pH | not recorded |
| Family | petase_like |
Structure
| Source | Experimental, PDB 7QVH |
| Resolution | 2.24 Å |
| Residues | 265 |
| Cα RMSD to IsPETase | 0.46 Å |
Active site
| Catalytic triad | Ser160 · His237 · Asp206 |
| Ser OG → His NE2 | 2.81 Å |
| His ND1 → Asp OD | 3.27 Å |
| Oxyanion donor 1 | 161 (2.79 Å) |
| Oxyanion donor 2 | 87 (4.75 Å) |
| Cleft width | 17.49 Å |
| Cleft depth | 4.16 Å |
| Cleft residues | 81 |
Aromatic clamp: PHE201 · TRP159 · TRP185 · TYR214 · TYR87
Mutations
Sequence recovered from a deposited construct rather than derived from a mutation list.
Sequence taken from the deposited construct 7QVH: 21 substitutions against IsPETase.
Reference: Bell et al. 2022, Nat. Catal. doi:10.1038/s41929-022-00821-3
Measured activity
| Parameter | Value | Substrate | Evidence | Source |
|---|---|---|---|---|
| performance claim | — | PET | from review | 10.1038/s41929-022-00821-3 |
| 21 mutations; melting temperature 82.5 °C | ||||
| topt | 62.5 degC | PET | from review | 10.1038/s41929-022-00821-3 |
| Published as 60 to 65 °C. Midpoint stored in the numeric column; the published interval is this text. | ||||
Related in PANTS
Lineage
Engineered from IsPETase.
Nearest metagenomic candidates
No candidate in the catalogue names this enzyme as its nearest match.
Identifiers and cross-references
Sequence taken from the deposited construct 7QVH, not derived from a mutation list: the PDB SEQRES is what was actually expressed, crystallised and assayed. 21 substitutions vs IsPETase, matching the published 21. Mature construct, so shorter than the precursor parent.