IsPETase
Piscinibacter sakaiensis · 290 aa · UniProt
Wild type; weak on crystalline PET
Structure, superposed on IsPETase
Aligned onto IsPETase 6EQE when it was written, so anything added below overlays directly and the browser does no alignment. The catalytic triad is drawn from the residues the geometry stage measured, not from positions inferred by an alignment.
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Sequence 290 aa
catalytic triad · the same colours as the viewer above. Triad positions are labelled; hover any residue for its number. Click one to select it in both.
Activity
| Optimum temperature | 40.0 °C |
| As published | Optimum temperature is 40 degrees Celsius for PET film hydrolysis (PubMed:26965627). Optimum temperature is 30 degrees Celsius for PET (commercial drinking bottle) hydrolysis and BHET hydrolysis (PubMed:29603535). Optimum temperature is 35-45 degrees Celsius for the hydrolysis of pNP-esters. Remains active even at 65 degrees Celsius (about 60% of maximum activity) (PubMed:30502092). |
| Optimum pH | 9.0 |
| Family | petase_like |
Structure
| Source | Experimental, PDB 6EQE |
| Resolution | 0.92 Å |
| Residues | 265 |
| Cα RMSD to IsPETase | 0.00 Å |
Active site
| Catalytic triad | Ser160 · His237 · Asp206 |
| Ser OG → His NE2 | 2.94 Å |
| His ND1 → Asp OD | 3.07 Å |
| Oxyanion donor 1 | 161 (3.29 Å) |
| Oxyanion donor 2 | 87 (5.65 Å) |
| Cleft width | 21.27 Å |
| Cleft depth | 4.27 Å |
| Cleft residues | 83 |
Aromatic clamp: PHE201 · TRP159 · TRP185 · TYR219 · TYR87
Measured activity
| Parameter | Value | Substrate | Evidence | Source |
|---|---|---|---|---|
| catalytic activity | — | PET | measured | PMID 26965627 · PMID 29235460 · PMID 29374183 · PMID 29603535 · PMID 29666242 · PMID 32269349 |
| (ethylene terephthalate)(n) + H2O = (ethylene terephthalate)(n-1) + 4-[(2-hydroxyethoxy)carbonyl]benzoate + H(+) | ||||
| km | 0.048 mM | pNP-octanoate | measured | PMID 30502092 |
| km | 0.053 mM | pNP-hexanoate | measured | PMID 30502092 |
| km | 0.315 mM | pNP-butanoate | measured | PMID 30502092 |
| km | 0.431 mM | pNP-acetate | measured | PMID 30502092 |
| km | 2.283 mM | pNP-dodecanoate | measured | PMID 30502092 |
| performance claim | — | PET | from review | 10.1126/science.aad6359 |
| Wild type; weak on crystalline PET | ||||
| ph opt | 9.0 pH | — | measured | PMID 26965627 · PMID 29603535 · PMID 30502092 |
| Optimum pH is 9 for PET film hydrolysis (PubMed:26965627). Optimum pH is 9 for PET (commercial drinking bottle) hydrolysis. Optimum pH is 6.5-8.0 for BHET hydrolysis (PubMed:29603535). Optimum pH is 8.0 for the hydrolysis of pNP-esters. The enzyme is active at pH 6-10, has an optimal pH range of 7-9 and it is rapidly inactivated below pH 7.0 or above pH 9.0 (PubMed:30502092). | ||||
| topt | 32.5 degC | PET | from review | 10.1126/science.aad6359 |
| Published as 30 to 35 °C. Midpoint stored in the numeric column; the published interval is this text. | ||||
| topt | 40.0 degC | — | measured | PMID 26965627 · PMID 29603535 · PMID 30502092 |
| Optimum temperature is 40 degrees Celsius for PET film hydrolysis (PubMed:26965627). Optimum temperature is 30 degrees Celsius for PET (commercial drinking bottle) hydrolysis and BHET hydrolysis (PubMed:29603535). Optimum temperature is 35-45 degrees Celsius for the hydrolysis of pNP-esters. Remains active even at 65 degrees Celsius (about 60% of maximum activity) (PubMed:30502092). | ||||
Related in PANTS
Lineage
Variants built on this enzyme:
DepoPETaseDuraPETaseESTHER:P71505FAST-PETaseFAST-PETase-N212A/K233C/S282CHotPETaseIsPETase-W159H/S238FThermoPETaseZ1-PETase
Nearest metagenomic candidates
No candidate in the catalogue names this enzyme as its nearest match.
Identifiers and cross-references
Sequence
Ideonella/Piscinibacter sakaiensis 201-F6. The reference PETase. 290 aa precursor; literature mutation numbering matches this precursor directly (verified: S160/D206/H237 triad, mobile W185).